Coding

Part:BBa_K4585003:Design

Designed by: Siyao Ma   Group: iGEM23_CSU-CHINA   (2023-09-22)


KRAB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 178


Design Notes

Different KRAB domains have different silencing effects on target genes, regardless of cell types. The KRAB protein domain must be linked to the DNA binding domains to play an inhibitory role.


Source

KRAB belongs to the N-terminal domain of zinc finger protein and comes from Lepeophtheirus Samis.

References